|
KMID : 0369819910210020067
|
|
Jorunal of Korean Pharmaceutical Sciences
1991 Volume.21 No. 2 p.67 ~ p.71
|
|
|
Quantitative Determination of Total Bile Acids from Bezoar and Bezoar - containing Liquid Preparation by Enzymatic Technique
|
|
하인식/Ha IS
김승환/차봉진/권종원/양중익/민신홍/Kim SH/Cha BJ/Kwon JW/Yang JI/Min SH
|
|
|
Abstract
|
|
|
|
A simple and sensitive method was developed for the quantification of free and conjugated bile acids in bezoar without prior hydrolysis. 3α?Hydroxy bile acids are first oxidized to 3-keto bile acids in the reaction catalyzed by 3α?hydroxysteroid dehydrogenase(3α?HSD). During this oxidative reaction, an equimolar quantity of nicotinamide adenine dinucleotide(NAD) is reduced to NADH and subsequently oxidized to NAD with concomitant reduction of nitrotetrazolium blue(NTB) to diformazan by the catalytic action of diaphorase. The diformazan has an absorbance maximum at 540 nm. The intensity of the color produced is directly proportional to bile acids concentration in the bezoar extracts. The optimum conditions for the enzymatic reaction such as effects of reaction time, reaction temperature and pH, and stability were investigated. Calibration plots for the sodium chelate observed to be linear and intra-, inter-assay analytical recovery of bile acids averaged 97.65±3.4. Therefore, it is considered that the quality control of total bile acids from bezoar or bezoar-containing liquid preparation using this simple and sensitive assay system will be acceptable. Also current bezoars and bezoar-containing liauid preparations were examined their total bile acids from this method.
|
|
|
KEYWORD
|
|
|
Bezoar, bile acids, 3α?hydroxysteroid dehydrogenase, enzymatic technique
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|